Isolation of the lipid-transporting protein Ns-LTP1 from seeds of the garden fennel flower (Nigella sativa)
Yu. I. OshchepkovaSadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Uzbek Republic, ul. Mirzo-Ulugbeka 83, Tashkent, 100125, UzbekistanO. N. VeshkurovaSadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Uzbek Republic, ul. Mirzo-Ulugbeka 83, Tashkent, 100125, UzbekistanЕ. А. РогожинShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, RussiaAlexander Kh. MusolyamovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, RussiaА. В. СмирновRussian State Agricultural University-Timiryazev Agricultural Academy, Moscow, RussiaТ. И. ОдинцоваVavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, RussiaTs. A. EgorovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, RussiaEugene V. GrishinShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, RussiaШ. И. СалиховSadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Uzbek Republic, ul. Mirzo-Ulugbeka 83, Tashkent, 100125, Uzbekistan
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A novel lipid-transporting protein (Ns-LTP1) has been isolated from seeds of the garden fennel flower Nigella sativa. The molecular mass, N-terminal amino acid sequence, and amino acid composition of the protein have been determined. Ns-LTP1 has a molecular mass of 9602 Da and contains eight cysteine residues which form four disulfide bridges. The protein is capable of suppressing the development of some phytopathogenic fungi and oomycetes.
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