Tissue culture of the Bud Organ from Litsea cubeba

Annotatsiya

We used one year old buds on stem of adult Litsea cubeba as explant tissue to make rapid propagation of the seedlings.The basic medium for Litsea cubeba tissue culture was recommended as an improved Ms(MS+Ca(NO3)2 380mg·L-1+H3BO3 8.2 mg·L-1),the proliferating culture was an improved Ms+BA 1.5 mg·L-1+IBA 0.2 mg·L-1+VC 10 mg·L-1+ sucrose 10 000 mg·L-1.On the condition of 2 000 ~ 4 000 lux natural scatted light,the period of sub-culture of GLLC-4,GLLC-3 and GLLC-1 secondary buds was 28 d,and the multiplication coefficient were 5.6,3.1 and 4.8 respectively.

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