Evaluation of the new, easy to perform, IS6110 based molecular assay Speed-oligo® Direct MTB (Vircell, Spain) for the detection of M. tuberculosis and direct comparison to probeTec (BD, USA)
Uladzimir AntonenkaIML Red GmbH, Synlab Bayern, Asklepios Fachklinken, WHO Supranationales Referenzlabor für Tuberkulose, GautingSabine Hofmann-ThielIML Red GmbH, Synlab Bayern, Asklepios Fachklinken, WHO Supranationales Referenzlabor für Tuberkulose, GautingA EsenalievaNational Reference Laboratory of Tuberculosis, Bishkek, Republic of KyrgyzstanLaziz TuraevNational Reference Laboratory of Tuberculosis, Tashkent, Republic of Uzbekistan National Institute of TuberculosisK MirazimNational Reference Laboratory of Tuberculosis, Tashkent, Republic of Uzbekistan National Institute of TuberculosisM AbdullaevaHarald HoffmannIML Red GmbH, Synlab Bayern, Asklepios Fachklinken, WHO Supranationales Referenzlabor für Tuberkulose, Gauting
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Background: “Speed-oligo® direct mycobacterium tuberculosis” (SPO DMTB) from Vircell is a novel assay, based on multiplex PCR dipstick hybridisation detecting Mycobacterium spp. and Mycobacterium tuberculosis complex (MTBC) in respiratory specimens. The amplification targets are: IS6110 (MTBC-specific), 16S rRNA (Mycobacterium genus-specific), and human gene RNAsaP (amplification control). Several authors consider IS6110-PCR as the most sensitive assay for direct detection of tuberculous bacteria in clinical specimens. We compare the IS6110 based SPO DMTB assay with ProbeTecTM MTBC (Becton Dickinson), another assay using the same target.
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