Separation,Purifi cation and Inhibitory Effect on Protein Glycosylation of Phenols in Kalimeris

Macroporous resin was used to separate the crude extract of Kalimeris and four polyphenol-rich fractions(F1,F2,F3,and F4) were obtained.F2 was further purifi ed by Sephadex LH-20 column chromatography and 3,5-dicaffeoylquinic acid and 3,4,5-tricaffeoylquinic acid were obtained.Using the protein glycosylation reaction models of bovine serum albumin-methylglyoxal(BSA-MGO) and bovine serum albumin-glyoxal(BSA-GO),we analyzed the inhibitory activity of each component on protein glycosylation.The results showed that the content of polyphenols was in the order of F2 F1 F3 F4,and all the four fractions had good inhibitory effects on protein glycosylation in both models.In BSA-GO reaction model,the inhibitory capacity against protein glycosylation was in the order of 3,5-dicaffeoylquinic acid F2 F3 F1 crude extract of Kalimeris F4.In BSA-MGO reaction model,the inhibitory capacity against protein gly cosylation was in the order of 3,5-dicaffeoylquinic acid F2 crude extract of Kalimeris F1 F4 F3.These results are consistent with the inhibitory effect of 3,5-dicaffeoylquinic acid separated from F2.The 3,5-dicaffeoylquinic acid as a phenolic compound from Kalimeris had a potent inhibitory effect on protein glycosylation induced by MGO or GO.

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