Preparative Separation of Lappaconitine from Aconitum leucostomum by HSCCC
Ayupbec AmatjanUsmanova SlukhanXinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, the Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Urumqi 830011, ChinaLi CXinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, the Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Urumqi 830011, ChinaKe Lin HUXinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, the Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Urumqi 830011, ChinaHaji Akber AisaXinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, the Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Urumqi 830011, China
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This paper describes a system for the preparation of lappaconitine from Aconitum leucostmum Worosch., using HSCCC, and examines the effect of the two-immiscible solvent system (TISS) composed of chloroform-methanol-0.2 mol/L hydrochloric acid (4:1.5:2). Under the optimized condition, about 75 mg of lappaconitine, at a purity of 98% that was done by high performance liquid chromatography (HPLC), are obtained from 250 mg of the crude alkaloid extract. The compound was checked by HPLC-UV, electrospray ionization mass spectrometry (ESI-MS), 1 H NMR and 13 C NMR.
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