Study self-transmissibility of Salmonella Typhimurium plasmids virulence isolated from fresh chicken foods by conjugation

The aim of the present investigation was experimentally the ability of the virulenceplasmid of Salmonella Typhimurium isolated from fresh chicken foods in al- Najaf Al-ashrafgovernorate to transfer between different isolates which might be the cause of variant diseasesin order to find new data about this objective that may help in study the control of infectionsin humans.S. typhimurium Lst1, Mst3 and Gst1 isolates ability to transfer t0 the transconjugant thenPCR results confirmed the frequency of spvA, pefA, rck and traT genes in E.colitransconjugant (TLst1) were (12%), (30%), (8%) and (26%) respectively where in (TMst1)transconjugant were(14%),(30%), (6%) and(28%) respectively, similar results appeared in(TGst1) transconjugant that were (6%),(17%), (4%)and (18%) respectively therefore presentstudy revealed according to conjugation experiments results that the virulence plasmid of S.typhimurium Lst1, Mst3 and Gst1 isolates were transmissible and perhaps lead to rapidincrease in virulence isolates among different bacterial species in Najaf governorate.

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