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Biomimetic engineering of the cardiac tissue through processing, functionalization, and biological characterization of polyester urethanes

Federico VozziInstitute of Clinical Physiology, IFC-CNR, Via Moruzzi 1, I-56124 Pisa,Federica LograndUniversity of TurinManuela CabiatiInstitute of Clinical Physiology, IFC-CNR, Via Moruzzi 1, I-56124 Pisa,Claudia CicioneUniversità Campus Bio-Medico di RomaMonica BoffitoDepartment of Mechanical and Aerospace Engineering, Polytechnic of Turin, Corso Duca degli Abruzzi 24, I-10129 Turin,Irene CarmagnolaDepartment of Mechanical and Aerospace Engineering, Polytechnic of Turin, Corso Duca degli Abruzzi 24, I-10129 Turin,Nicoletta VitaleUniversity of TurinManuele GoriUniversità Campus Bio-Medico di RomaMara BrancaccioUniversity of TurinSilvia Del RyInstitute of Clinical Physiology, IFC-CNR, Via Moruzzi 1, I-56124 Pisa,Dario GastaldiDepartment of Chemistry, Materials and Chemical Engineering ‘Giulio Natta’, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milan,Emanuele CattarinuzziDepartment of Chemistry, Materials and Chemical Engineering ‘Giulio Natta’, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milan,Pasquale VenaDepartment of Chemistry, Materials and Chemical Engineering ‘Giulio Natta’, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milan,Alberto RainerUniversità Campus Bio-Medico di RomaClaudio DomeniciInstitute of Clinical Physiology, IFC-CNR, Via Moruzzi 1, I-56124 Pisa,Gianluca CiardelliDepartment of Mechanical and Aerospace Engineering, Polytechnic of Turin, Corso Duca degli Abruzzi 24, I-10129 Turin,Susanna SartoriDepartment of Mechanical and Aerospace Engineering, Polytechnic of Turin, Corso Duca degli Abruzzi 24, I-10129 Turin,

Biomedical Materialsjournal2018en

ABI

Annotatsiya

Three-dimensional (3D) tissue models offer new tools in the study of diseases. In the case of the engineering of cardiac muscle, a realistic goal would be the design of a scaffold able to replicate the tissue-specific architecture, mechanical properties, and chemical composition, so that it recapitulates the main functions of the tissue. This work is focused on the design and preliminary biological validation of an innovative polyester urethane (PUR) scaffold mimicking cardiac tissue properties. The porous scaffold was fabricated by thermally induced phase separation (TIPS) from poly(ε-caprolactone) diol, 1,4-butanediisocyanate, and l-lysine ethyl ester. Morphological and mechanical scaffolds characterization was accomplished by confocal microscopy, and micro-tensile and compression techniques. Scaffolds were then functionalized with fibronectin by plasma treatment, and the surface treatment was studied by x-ray photoelectron spectroscopy, attenuated total reflectance Fourier transform infrared spectra, and contact angle measurements. Primary rat neonatal cardiomyocytes were seeded on scaffolds, and their colonization, survival, and beating activity were analyzed for 14 days. Signal transduction pathways and apoptosis involved in cells, the structural development of the heart, and its metabolism were analyzed. PUR scaffolds showed a porous-aligned structure and mechanical properties consistent with that of the myocardial tissue. Cardiomyocytes plated on the scaffolds showed a high survival rate and a stable beating activity. Serine/threonine kinase (AKT) and extracellular signal-regulated kinases (ERK) phosphorylation was higher in cardiomyocytes cultured on the PUR scaffold compared to those on tissue culture plates. Real-time polymerase chain reaction analysis showed a significant modulation at 14 days of cardiac muscle (MYH7, prepro-ET-1), hypertrophy-specific (CTGF), and metabolism-related (SLC2a1, PFKL) genes in PUR scaffolds.

Mavzular

Tissue Engineering and Regenerative Medicine Electrospun Nanofibers in Biomedical Applications Cellular and Composite Structures

Identifikatorlar

DOI: 10.1088/1748-605x/aaca5b

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