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The Interaction of Sio <sub>2</sub> Nanoparticles with The Neuronal Cell Membrane: Activation of Ionic Channels and Calcium Influx

Carla DistasiDepartment of Pharmaceutical Sciences, University of Piemonte Orientale ‘A. Avogadro’, Via Bovio 6, 28100Novara, ItalyMarianna DionisiDepartment of Pharmaceutical Sciences, University of Piemonte Orientale ‘A. Avogadro’, Via Bovio 6, 28100Novara, ItalyFederico Alessandro RuffinattiDepartment of Pharmaceutical Sciences, University of Piemonte Orientale ‘A. Avogadro’, Via Bovio 6, 28100Novara, ItalyAlessandra GilardinoDepartment of Life Sciences & Systems Biology, University of Torino, via Accademia Albertina 23, 10123Torino, ItalyRoberta BardiniDepartment of Life Sciences & Systems Biology, University of Torino, via Accademia Albertina 23, 10123Torino, ItalySusanna AntoniottiDepartment of Life Sciences & Systems Biology, University of Torino, via Accademia Albertina 23, 10123Torino, ItalyFederico CatalanoDepartment of Chemistry, Torino, University of Torino, Via P. Giuria 9, 10125, ItalyEleonora BassinoDepartment of Life Sciences & Systems Biology, University of Torino, via Accademia Albertina 23, 10123Torino, ItalyLuca MunaronDepartment of Life Sciences & Systems Biology, University of Torino, via Accademia Albertina 23, 10123Torino, ItalyGianmario MartraDepartment of Control & Computer Engineering, Polytechnic University of Turin, Corso Duca degli Abruzzi 24, 10129Torino, ItalyDavide LovisoloDepartment of Life Sciences & Systems Biology, University of Torino, via Accademia Albertina 23, 10123Torino, Italy

Nanomedicinejournal2019en

ABI

Annotatsiya

Aim: To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1–7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials & methods: Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+]i increase. Conclusion: These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.

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Mavzular

Connexins and lens biology Biochemical effects in animals Ion Channels and Receptors

Identifikatorlar

DOI: 10.2217/nnm-2018-0256

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