Features of seed growth and development of sproutings of the russian dereza (Lycium ruthenicum Murr.) under laboratory conditions and in culture in vitro

Russian dereza (Lycium ruthenicum Murr.) is a shrub plant that spreads on the territory of the dried Aral Sea in conditions of high soil salinity, dry and sharply continental climate. The fruits were collected and the features of germination of seeds of the russian dereza halophyte (Lycium ruthenicum Murr.) Under sterile and non-sterile conditions were studied. The optimal temperature and illumination regime for the germination of annual seeds of this species is a 16-hour photoperiod and a temperature of 25 °C, the germination of seeds in nonsterile conditions was: 4-year-old – 46 %; 2-year – 83; one-year – 96 %. It has been shown that L. ruthenicum plants remain viable for up to 4 years or more, on this basis, we attributed the seeds of this species to truly orthodox. When introduced into culture in vitro, it has been shown that multi-stage sterilization significantly reduces the viability of seeds and seedlings of Lycium ruthenicum Murr., which leads to a decrease in seed germination by up to 40 %. The optimal nutrient medium for the stable development of microshoots without anomalies, callus formation and initiation of root formation was MS with the addition of 1.0 mg/l 6-BAP to the nutrient medium, without sucrose. Maintaining the samples in the in vitro collection is carried out on a half-MS medium without hormones, without sucrose at a low positive temperature of 4 °C, illumination of ~500 lx and a photoperiod of 8 hours. The samples of L. ruthenicum in vitro can be used to develop methods of clonal micropropagation; for genotyping of samples and identification of molecular biomarkers of plant resistance to soil salinity; in the ex vitro morpho-biological study of plants resistant to salt stress.

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