Biological control of Fusarium oxysporum in shallot wilt disease using arbuscular mycorrhiza combined with Trichoderma sp., Pseudomonas sp., and Bacillus sp.

This study aimed to control Fusarium wilt disease in shallots using arbuscular mycorrhizal fungi, Trichoderma sp. and Bacillus sp.The research was carried out at the Screen House and Plant Protection Laboratory.The research design used a completely randomized (CRD) method with five treatments repeated four times with three experimental units in each treatment.The treatments were arbuscular mycorrhiza (M), soaking seeds with PGPM (So), Spraying with PGPM (S), arbuscular mycorrhiza + soaking seeds (MSo), and arbuscular mycorrhiza + soaking PGPM + spraying PGPM (MSoS), and control (C).Observation variables included the incubation period of Fusarium wilt disease, the incidence of Fusarium wilt disease, and plant products consisting of the number of leaves, plant height, number of tubers, and tuber weight.The results show that the fastest incubation period was four days after the inoculation of the Fusarium in the control treatment.The treatment of seed soaking, spraying and application of arbuscular mycorrhizae (RSM) showed the longest incubation period, 14 days.The RSM treatment able to suppress the Fusarium wilts disease more than 50%.The shallot growth performance and production increase accordingly.

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