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Real-time microbial growth curve (RMGC) system: an improved microplate reader with a graphical interface for automatic and high-throughput monitoring of microbial growth curves

Yongjie ZhongSchool of Information and Communication Engineering, Marine College, School of Biomedical Engineering, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaZhuoyuan LaiSchool of Information and Communication Engineering, Marine College, School of Biomedical Engineering, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaChanghua HeDepartment of Public Health, Hainan Provincial Center for Disease Control and Prevention, No. 40, Haifu Road, Meilan district, Haikou city, Hainan Province, ChinaShengsen PengSchool of Information and Communication Engineering, Marine College, School of Biomedical Engineering, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaTianci GuoSchool of Information and Communication Engineering, Marine College, School of Biomedical Engineering, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaHui YangSchool of Information and Communication Engineering, Marine College, School of Biomedical Engineering, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaFan YangSchool of Information and Communication Engineering, Marine College, School of Biomedical Engineering, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaYi ShenSchool of Information and Communication Engineering, Marine College, School of Biomedical Engineering, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaZhengliang HuangZhang FuK WangFengge SongSchool of Information and Communication Engineering, Marine College, School of Biomedical Engineering, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaJinghao YangInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, ChinaMasoud NegahdaryCenter for Remote Health Technologies & Systems, Texas A&M Engineering Experiment Station, 600 Discovery Drive, College Station, TX, 77840-3006, USAHaimei MaoHongliang ZhaoYi WanSchool of Information and Communication Engineering, Marine College, School of Biomedical Engineering, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaKhaydar E. YunusovDepartment of Cellulose and its Derivatives Chemistry and Technology, Institute of Polymer Chemistry and Physics, Uzbekistan Academy of Sciences, str. A. Khodiriy 7b, Tashkent, 100128, UzbekistanAbdushkur A. SarimsakovDepartment of Cellulose and its Derivatives Chemistry and Technology, Institute of Polymer Chemistry and Physics, Uzbekistan Academy of Sciences, str. A. Khodiriy 7b, Tashkent, 100128, Uzbekistan

The Analystjournal2025en

ABI

Annotatsiya

. After approximately 12 hours of continuous monitoring, the system exhibited a relative standard deviation (RSD) of less than 3.25% for optical density (OD) measurements and an RSD of 2.52% for the point of inflection (POI). These results indicate a similar level of precision but a longer monitoring time compared to conventional microplate readers, reflecting the effectiveness of the RMGC system in accurately monitoring microbial growth. The RMGC system showcases its versatility through various applications, such as microorganism gradient cultures, anaerobic microbial cultures, and antimicrobial susceptibility testing (AST). Its capabilities have important implications for multiple industries, including pharmaceuticals for antibiotic development, food safety for microbial contamination testing, and microbiological research.

Mavzular

Biosensors and Analytical Detection Microbial Community Ecology and Physiology

Identifikatorlar

DOI: 10.1039/d4an01339e

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