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Differential Analysis of Non-Volatile and Volatile Organic Compounds in Lonicerae japonicae Flos Across Four Geographical Origins of China Using HS-GC-IMS, HS-SPME-GC-MS, UPLC-Q-TOF-MS, and Multivariate Statistical Methods

Xiaobei NingKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaHeng LuKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaLili LiKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaMinmin ZhangKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaYujuan JiangShandong C.P. Freda Pharmaceutical Co., Ltd., Jinan 250100, ChinaIbragimov Aziz BakhtiyarovichInstitute of General and Inorganic Chemistry of Uzbekistan Academy of Sciences, Mirzo Ulug’bek Str., 77a, Tashkent 100170, UzbekistanXiao WangKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaIftikhar AliDepartment of Chemistry, Karakoram International University, Gilgit 15100, PakistanWenhua JiKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China

Moleculesjournal2025en

ABI

Annotatsiya

flos (LJF). The present research was designed to explore differences among volatile organic compounds (VOCs) and non-VOCs among LJF samples from four geographical origins. Selection of 32 LJF samples with similar genetic backgrounds was performed using simple sequence repeat markers. Headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) and headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) were utilized to analyze VOCs, while non-VOCs were detected via ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Multivariate statistical analyses were applied to screen differential compounds. The results indicated that HS-SPME-GC-MS and HS-GC-IMS identified 80 and 57 VOCs, respectively, with 34 key differential VOCs screened out, exhibiting significant variations among origins. For non-VOCs, 130 compounds were identified, with 19 key differential compounds showing geographical differences. This study further facilitates a comprehensive understanding of the chemical composition of LJF from different origins.

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Mavzular

Fungal Biology and Applications Advanced Chemical Sensor Technologies Sesquiterpenes and Asteraceae Studies

Identifikatorlar

DOI: 10.3390/molecules31010004

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