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Methylome and Transcriptome Analysis Reveals Differences in Callus Development and Plantlet Regeneration Capacity Between Two Eucalyptus Species

B. ChenGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaChunyan GanGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaShengkan ChenGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaDongqiang GuoGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaGuichan LiangGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaXiaoying FangGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaHui ZhuGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaZiyu DengGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaQinglan TangGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaYufei XiaoGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, ChinaChunjie FanResearch Institute of Tropical Forestry, Chinese Academy of Forestry, 682 Guangshan 1st Road, Guangzhou 510520, ChinaChangrong LiGuangxi Laboratory of Forestry, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, 23 Yongwu Road, Nanning 530002, China

Plantsjournal2026en

ABI

Annotatsiya

Eucalyptus is a highly diverse genus of the Myrtaceae family that is planted worldwide. Many changes occur during callus development, an important process during in vitro plant regeneration. In this study, we conducted methylome and transcriptome analyses to reveal such changes. The results showed that differentially expressed genes between E. camaldulensis (voucher ID: c0009; high embryogenic potential) and E. grandis × urophylla (voucher ID: j0017; low embryogenic potential) during callus development were enriched in plant hormone signal transduction and MAPK (Mitogen-activated protein kinase) signaling pathways. qRT-PCR analysis showed AHP, BAK1, BSK, CRE1, GID1, MKS1, PR-1, PYL, RbohD, and TCH4 could be involved in the callus development and plantlet regeneration capacity. The differences observed in regenerative potential during callus maturation between the two species under study provide a reliable molecular basis for the study of Eucalyptus regeneration mechanisms.

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Mavzular

Plant tissue culture and regeneration Plant Molecular Biology Research Plant Gene Expression Analysis

Identifikatorlar

DOI: 10.3390/plants15050783

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