Biosynthesis of Silver Nanoparticles by Indicator Microorganisms Isolated from Anthropogenically Impacted Soils

Nanotechnology has recently become increasingly widespread in various areas of human activity. Various methods for producing nanoparticles of metals and other substances are used worldwide. Microbial synthesis is the most environmentally friendly method. Soil is the richest source of microorganisms, and anthropogenic impacts on soil promote the development of resistance mechanisms in the microorganisms living there. In this study, we examined the ability of indicator bacteria newly isolated from contaminated urban soils to synthesize silver nanoparticles. Two isolates were identified as the most active silver nanoparticle producers. Molecular genetic identification revealed that these isolates belong to Bacillus halotolerans and Pseudarthrobacter oxydans. UV spectroscopy analysis revealed that the newly isolated bacteria synthesize silver nanoparticles measuring 10-40 nm. These results can be used for further research and the development of biopharmaceuticals for use in various areas of human life.

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