Multitarget Antithrombotic Effects of Scutellarin On Platelet–Coagulation Hemostasis

Background: Scutellarin, a flavonoid-rich compound derived from Erigeron breviscapus–related plant material, has been reported to exert cardiovascular and antithrombotic effects, but its integrated influence on platelet–coagulation hemostasis remains incompletely characterized. Methods: we analyzed the in vitro effects of scutellarin on thrombin time (TT), activated recalcification time (ART), activated partial thromboplastin time (APTT), prothrombin time (PT), and ADP-induced platelet aggregation, as well as its effects on intracellular Ca2+ signaling in platelets. Results: Scutellarin prolonged TT, ART, APTT, and PT in a concentration-dependent manner, indicating a broad anticoagulant profile spanning both intrinsic and extrinsic/common coagulation pathways. TT increased from a baseline range of 18–25 s to as high as 140 s at 50 μM, while PT reached approximately 85 s at the upper tested concentrations. In platelet studies, scutellarin strongly inhibited the second phase of ADP-induced aggregation and attenuated ADP-evoked intracellular Ca2+ rise by approximately 16–19% without behaving as a simple extracellular Ca2+ chelator. Platelet counts were not significantly reduced. Conclusions: The available experimental data support scutellarin as a multitarget modulator of platelet–coagulation hemostasis with both antiplatelet and anticoagulant properties. The pattern of response is consistent with interference at several levels, including thrombin/fibrin formation, coagulation-complex assembly on phosphatidylserine-rich membranes, and receptor-linked platelet Ca2+ signaling.

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