Improved DNA extraction from ancient bones using silica-based spin columns
Dongya YangDepartment of Anthropology, McMaster University, Hamilton, Ontario, CanadaBarry EngDepartment of Pathology, McMaster University, Hamilton, Ontario L8N 3Z5, CanadaJohn S. WayeDepartment of Pathology, McMaster University, Hamilton, Ontario L8N 3Z5, CanadaJ. Christopher DudarDepartment of Anthropology, McMaster University, Hamilton, Ontario L8S 4L9, CanadaShelley R. SaundersDepartment of Anthropology, McMaster University, Hamilton, Ontario L8S 4L9, Canada
1998en
ABI
Annotatsiya
We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.
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