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PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

OliverH. Lowrythe Department of Pharmacology, Washington University School oj Medicine, St. Louis, MissouriNiraJ. Rosebroughthe Department of Pharmacology, Washington University School oj Medicine, St. Louis, MissouriA. Farrthe Department of Pharmacology, Washington University School oj Medicine, St. Louis, MissouriRoseJ. Randallthe Department of Pharmacology, Washington University School oj Medicine, St. Louis, Missouri
1951zu
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Since 1922 when Wu proposed the use of the Folin phenol reagent for the measurement of proteins (l), a number of modified analytical procedures ut.ilizing this reagent have been reported for the determination of proteins in serum (2-G), in antigen-antibody precipitates (7-9), and in insulin (10).

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