A MODIFIED DNA EXTRACTION MINIPREPARATION PROTOCOL FOR <i>FUSARIUM</i> ISOLATES
Kamel A. Abd–ElsalamInstitute of Phytopathology Christian Albrechts University of Kiel Hermann-Rodewald-St. 9 D-24118, Kiel, GermanyF. SchniederInstitute of Phytopathology Christian Albrechts University of Kiel Hermann-Rodewald-St. 9 D-24118, Kiel, GermanyJianrong GuoInstitute of Phytopathology Christian Albrechts University of Kiel Hermann-Rodewald-St. 9 D-24118, Kiel, Germany
2003en
ABI
Annotatsiya
Abstract We have modified a quick, inexpensive and less prone to contamination protocol by culturing the cryopreserved or fresh mycelium directly in 96‐deepwell plate. The method facilitates concomitant assessment of ssDNA fungal diversity by asymmetric PCR (A‐PCR) from a single extraction. The DNA yields from Fusarium spp. isolates was reasonably high, and a clear DNA band was frequently seen when 10 μL of the PCR product was run in agarose gel. The procedure can be completed in less than 4 h and 96 samples can be processed at the same time.
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