An efficient approach for screening minimal PKS genes from Streptomyces
Mikko Metsä‐KeteläUniversity of Turku, Biochemistry, FIN-20014, Turku, FinlandVirpi SaloUniversity of Turku, Biochemistry, FIN-20014 Turku, FinlandLaura M. HaloUniversity of Turku, Biochemistry, FIN-20014 Turku, FinlandAnne HautalaGalilaeus Oy, Kairiskulmantie 10, FIN-20780 Kaarina, FinlandJuha HakalaGalilaeus Oy, Kairiskulmantie 10, FIN-20780 Kaarina, FinlandPekka MäntsäläUniversity of Turku, Biochemistry, FIN-20014 Turku, FinlandKristiina YlihonkoGalilaeus Oy, Kairiskulmantie 10, FIN-20780 Kaarina, Finland
1999en
ABI
Annotatsiya
Degenerated oligonucleotide primers were designed to amplify fragments of ketosynthase genes from polyketide antibiotics producing Streptomyces spp. and bacterial strains enriched from soil samples. Cell lysates were used as templates in amplification, so time-consuming DNA purification was avoided. A phylogenetic tree constructed from the amino acid sequences of the amplified fragments shows a distribution of spore pigments and antibiotics in separate classes. In addition, several different subgroups form within the antibiotics group. Anthracyclines were divided into separate branches according to the starter unit used in biosynthesis.
Hali tarjima qilinmagan
Identifikatorlar
Iqtiboslar va manbalar
2 ta iqtibos0 ta foydalanilgan manba