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Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand

Saowaluck TibprommaCentre of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, ThailandKevin D. HydeCentre of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, ThailandJayarama D. BhatFormerly, Department of Botany, Goa University, Taleigão, Goa, IndiaPeter E. MortimerKey Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, People's Republic of ChinaJianchu XuKey Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, People's Republic of ChinaItthayakorn PromputthaDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, ThailandMingkwan Doilom-J, Azad Housing Society, Curca, Goa Velha, IndiaJunbo YangGermplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, ChinaAlvin M. C. TangDivision of Applied Science, College of International Education, The Hong Kong Baptist University, Hong Kong SAR, ChinaSamantha C. KarunarathnaCentre of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand
2018en
ABI

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The authors established the taxonomic status of endophytic fungi associated with leaves of Pandanaceae collected from southern Thailand. Morphotypes were initially identified based on their characteristics in culture and species level identification was done based on both morphological characteristics and phylogenetic analyses of DNA sequence data. Twenty-two isolates from healthy leaves were categorised into eight morphotypes. Appropriate universal primers were used to amplify specific gene regions and phylogenetic analyses were performed to identify these endophytes and established relationships with extant fungi. The authors identified both ascomycete and basidiomycete species, including one new genus, seven new species and nine known species. Morphological descriptions, colour plates and phylogenies are given for each taxon.

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