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Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae

Rupert W. OsbornZeneca Agrochemicals, Bracknell, Berks, UKGenoveva W. De SamblanxF.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Willem De Croylaan 42, B-3001 Heverlee, BelgiumKarin ThevissenF.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Willem De Croylaan 42, B-3001 Heverlee, BelgiumInge J.W.M. GoderisF.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Willem De Croylaan 42, B-3001 Heverlee, BelgiumSophie TorrekensCenter of Human Genetics, Catholic University of Leuven, Willem De Croylaan 42, B-3001 Heverlee, BelgiumFred Van LeuvenCenter of Human Genetics, Catholic University of Leuven, Willem De Croylaan 42, B-3001 Heverlee, BelgiumSheila AttenboroughZeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berks, UKSarah B. ReesZeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berks, UKWillem F. BroekaertF.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Willem De Croylaan 42, B-3001 Heverlee, Belgium
1995en
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Annotatsiya

From seeds of Aesculus hippocastanum, Clitoria ternatea, Dahlia merckii and Heuchera sanguinea five antifungal proteins were isolated and shown to be homologous to plant defensins previously characterised from radish seeds and gamma-thionins from Poaceae seeds. Based on the spectrum of their antimicrobial activity and the morphological distortions they induce on fungi the peptides can be divided into two classes. The peptides did not inhibit any of three different alpha-amylases.

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