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Polylysine-decorated macroporous microcarriers laden with adipose-derived stem cells promote nerve regeneration in vivo

Yi SunQingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, PR ChinaXiaoqi ChiSchool of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR ChinaHaoye MengInstitute of Orthopedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Lab of Musculoskeletal Trauma & War Injuries, PLA, No.28 Fuxing Road, Beijing, 100853, PR ChinaMengjiao MaSchool of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR ChinaJing WangInstitute of Orthopedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Lab of Musculoskeletal Trauma & War Injuries, PLA, No.28 Fuxing Road, Beijing, 100853, PR ChinaZhaoxuan FengSchool of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR ChinaQi QuanInstitute of Orthopedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Lab of Musculoskeletal Trauma & War Injuries, PLA, No.28 Fuxing Road, Beijing, 100853, PR ChinaGuodong LiuSchool of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR ChinaYansen WangSchool of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR ChinaYajie XieSchool of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR ChinaYudong ZhengSchool of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR ChinaJiang PengCo-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, 226007, PR China

2021en

ABI

Annotatsiya

Cell transplantation is an effective strategy to improve the repair effect of nerve guide conduits (NGCs). However, problems such as low loading efficiency and cell anoikis undermine the outcomes. Microcarriers are efficient 3D cell culture scaffolds, which can also prevent cell anoikis by providing substrate for adhesion during transplantation. Here, we demonstrate for the first time microcarrier-based cell transplantation in peripheral nerve repair. We first prepared macroporous chitosan microcarriers (CSMCs) by the emulsion-phase separation method, and then decorated the CSMCs with polylysine (pl-CSMCs) to improve cell affinity. We then loaded the pl-CSMCs with adipose-derived stem cells (ADSCs) and injected them into electrospun polycaprolactone/chitosan NGCs to repair rat sciatic nerve defects. The ADSCs-laden pl-CSMCs effectively improved nerve regeneration as demonstrated by evaluation of histology, motor function recovery, electrophysiology, and gastrocnemius recovery. With efficient cell transplantation, convenient operation, and the multiple merits of ADSCs, the ADSCs-laden pl-CSMCs hold good potential in peripheral nerve repair.

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Identifikatorlar

DOI: 10.1016/j.bioactmat.2021.03.029

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