Maqola

Impairment of Biofilm Formation by TiO<sub>2</sub> Photocatalysis through Quorum Quenching

Xiang XiaoCAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei 230026, ChinaWenwen ZhuSchool of The Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, ChinaQiuyue LiuSchool of The Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, ChinaHang YuanKey Laboratory of Ion Beam Bioengineering, Institute of Technical Biology & Agriculture Engineering, Chinese Academy of Sciences, Hefei, 230031, ChinaWen‐Wei LiCAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei 230026, ChinaLijun WuKey Laboratory of Ion Beam Bioengineering, Institute of Technical Biology & Agriculture Engineering, Chinese Academy of Sciences, Hefei, 230031, ChinaQian LiSchool of The Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, ChinaHan‐Qing YuCAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei 230026, China

2016en

ABI

Annotatsiya

The release of nanomaterials into the environment, due to their massive production and application today, has caused ecological and health safety concerns. Semiconductor photocatalysts like TiO2 exhibit cytotoxicity to bacterial cells when exposed to UV irradiation. However, information about their impacts on individual or group bacterial behaviors is limited. In this work, the biofilm formation of Escherichia coli K12 in the presence of TiO2 with and without UV irradiation was investigated and biofilm formation was found not to be affected under the sole application of TiO2 or UV irradiation. However, biofilm development was substantially delayed by TiO2 under UV irradiation, although no obvious cytotoxicity to cell growth was observed. The reactive oxygen species photogenerated by TiO2 were found to quench the autoinducer 2 (AI-2) signals secreted by E. coli K12. As a result, the initiation of quorum sensing for biofilm formation activated by AI-2 was restrained. The expressions of two biofilm-formation-related genes, motA and rcsB, were also suppressed. A dose of an AI-2 precursor, 4,5-dihydroxy-2,3-pentanedione, effectively restored the biofilm development. These results show that the photoexcited TiO2 could suppress biofilm formation through quenching AI-2 signals. This work may facilitate a better understanding about the ecological effects of increasingly released nanomaterials and provide implications for development of antifouling membranes.

Hali tarjima qilinmagan

Identifikatorlar

DOI: 10.1021/acs.est.6b03134

Iqtiboslar va manbalar

2 ta iqtibos0 ta foydalanilgan manba

Koʻrsatkichlar — AkademScholar