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3D bioprinting of collagen to rebuild components of the human heart

Andrew LeeDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USAAndrew R. HudsonDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USADaniel J. ShiwarskiDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USAJoshua W. TashmanDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USAThomas J. HintonDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USASaigopalakrishna S. YerneniDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USAJacqueline M. BlileyDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USAPhil G. CampbellDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USAAdam W. FeinbergDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA

2019en

ABI

Annotatsiya

Collagen is the primary component of the extracellular matrix in the human body. It has proved challenging to fabricate collagen scaffolds capable of replicating the structure and function of tissues and organs. We present a method to 3D-bioprint collagen using freeform reversible embedding of suspended hydrogels (FRESH) to engineer components of the human heart at various scales, from capillaries to the full organ. Control of pH-driven gelation provides 20-micrometer filament resolution, a porous microstructure that enables rapid cellular infiltration and microvascularization, and mechanical strength for fabrication and perfusion of multiscale vasculature and tri-leaflet valves. We found that FRESH 3D-bioprinted hearts accurately reproduce patient-specific anatomical structure as determined by micro-computed tomography. Cardiac ventricles printed with human cardiomyocytes showed synchronized contractions, directional action potential propagation, and wall thickening up to 14% during peak systole.

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Identifikatorlar

DOI: 10.1126/science.aav9051

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