Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents
Brian F. MeyerDepartment of Medicinal Chemistry and Pharmacognosy, School of Pharmacy and Pharmacal Sciences, and Cell Culture Laboratory, Purdue Cancer Center, Purdue University, West Lafayette, IN 47907N. R. FerrigniDepartment of Medicinal Chemistry and Pharmacognosy, School of Pharmacy and Pharmacal Sciences, and Cell Culture Laboratory, Purdue Cancer Center, Purdue University, West Lafayette, IN 47907J. E. PutnamDepartment of Medicinal Chemistry and Pharmacognosy, School of Pharmacy and Pharmacal Sciences, and Cell Culture Laboratory, Purdue Cancer Center, Purdue University, West Lafayette, IN 47907Linda JacobsenDepartment of Medicinal Chemistry and Pharmacognosy, School of Pharmacy and Pharmacal Sciences, and Cell Culture Laboratory, Purdue Cancer Center, Purdue University, West Lafayette, IN 47907D. E. NICHOLSDepartment of Medicinal Chemistry and Pharmacognosy, School of Pharmacy and Pharmacal Sciences, and Cell Culture Laboratory, Purdue Cancer Center, Purdue University, West Lafayette, IN 47907J. McLaughlinDepartment of Medicinal Chemistry and Pharmacognosy, School of Pharmacy and Pharmacal Sciences, and Cell Culture Laboratory, Purdue Cancer Center, Purdue University, West Lafayette, IN 47907
1982en
ABI
Annotatsiya
A method, utilizing brine shrimp (Artemia salina Leach), is proposed as a simple bioassay for natural product research. The procedure determines LC (50) values in microg/ml of active compounds and extracts in the brine medium. Activities of a broad range of known active compounds are manifested as toxicity to the shrimp. Screening results with seed extracts of 41 species of Euphorbiaceae were compared with 9KB and 9PS cytotoxicities. The method is rapid, reliable, inexpensive, and convenient as an in-house general bioassay tool.
Hali tarjima qilinmagan
Identifikatorlar
Iqtiboslar va manbalar
2 ta iqtibos0 ta foydalanilgan manba