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Flavonoid Determination in the Quality Control of Floral Bioresidues from <i>Crocus sativus</i> L.

Jéssica Serrano‐DíazCátedra de Quı́mica Agrı́cola, ETSI Agrónomos, Universidad de Castilla-La Mancha, Campus Universitario, 02071 Albacete, SpainAna M. SánchezInstitut für Lebensmittelchemie, Technische Universität Braunschweig, 38106 Braunschweig, Schleinitzstrasse 20, GermanyMagdalena Martínez‐ToméFood Technology, Nutrition and Food Science Department, Regional Campus of International Excellence “Campus Mare Nostrum”, Murcia University, 30100 Murcia, SpainPeter WinterhalterInstitut für Lebensmittelchemie, Technische Universität Braunschweig, 38106 Braunschweig, Schleinitzstrasse 20, GermanyGonzalo L. AlonsoCátedra de Quı́mica Agrı́cola, ETSI Agrónomos, Universidad de Castilla-La Mancha, Campus Universitario, 02071 Albacete, Spain

2014en

ABI

Annotatsiya

A high-performance liquid chromatography with photodiode array detection method (HPLC-DAD) was validated for the analysis of floral bioresidues obtained in saffron spice production by using three different solvent mixtures [water/hydrochloric acid (HCl) (100:1, v/v), water/acetonitrile/trifluoroacetic acid (47:50:3, v/v/v), and water/acetonitrile/HCl (50:50:1, v/v/v)]. Fifteen phenolic compounds were tentatively identified, kaempferol 3-O-sophoroside and delphinidin 3,5-di-O-glucoside being the main ones. The extracts showed very different phenolic profiles obtained by HPLC-DAD coupled with electrospray ionization mass spectrometry (ESI-MSn), and several experiments were carried out to explain this. The use of acetonitrile as solvent causes the chromatographic splitting of the peak of the delphinidin 3,5-di-O-glucoside into two peaks. Results obtained in this paper show that the extract prepared with water/HCl (100:1, v/v) would be the best suited for determining phenolic compounds in the quality control of the floral bioresidues from Crocus sativus L.

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DOI: 10.1021/jf4057023

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