Maqola

Specialized scanning ion‐conductance microscope for imaging of living cells

Yuri E. KorchevDepartment of Physiology, Charing Cross and Westminster Medical School, University of London, U.K. [email protected]M. MilovanovićC.L. BashfordDorothy C. BennettDepartment of Anatomy and Developmental Biology, St George's Hospital Medical School, University of London, Cranmer Terrace, London SW17 0RE, U.K.,Elena V. SviderskayaDepartment of Anatomy and Developmental Biology, St George's Hospital Medical School, University of London, Cranmer Terrace, London SW17 0RE, U.K.,Igor VodyanoyDepartment of Physiology, Charing Cross and Westminster Medical School, University of London, St Dunstan's Road, London W6 8RP, U.K.,M J LabDepartment of Physiology, Charing Cross and Westminster Medical School, University of London, St Dunstan's Road, London W6 8RP, U.K.,

1997en

ABI

Annotatsiya

A specialized scanning ion conductance microscope (SICM) for imaging living cells has been developed from a conventional patch-clamp apparatus, which uses a glass micropipette as the sensitive probe. In contrast with other types of scanning probe microscope, the SICM probe has significant advantages for imaging living cells: it is most suitable for imaging samples immersed in water solutions; and since the probe senses ion current and does not need physical contact with the sample during the scan, any preliminary preparation of cells (fixation or adherence to a substrate) is unnecessary. We have successfully imaged murine melanocytes in growth medium. The microscope images the highly convoluted surface structures without damaging or deforming them, and reveals the true, three-dimensional relief of the cells. This instrument has considerable ability to operate, potentially simultaneously, in applications as diverse as real-time microscopy, electrophysiology, micromanipulation and drug delivery.

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Identifikatorlar

DOI: 10.1046/j.1365-2818.1997.2430801.x

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