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Two non-vesicular ATP release pathways in the mouse erythrocyte membrane

Feng QiuDepartment of Physiology and Biophysics, University of Miami, School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USAJunjie WangDepartment of Physiology and Biophysics, University of Miami, School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USADavid C. SprayThe Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USAEliana ScemesThe Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USAGerhard DahlDepartment of Physiology and Biophysics, University of Miami, School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USA

2011en

ABI

Annotatsiya

Erythrocytes are exceptionally suited for analysis of non-exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here we show that erythrocytes of Panx1(-/-) mice lend further support to this conclusion. However, ATP release, although attenuated, was still observed in Panx1(-/-) mouse erythrocytes. In contrast to Panx1(+/+) cells, this release was not correlated with uptake of extracellularly applied dyes, was insensitive to Panx1 channel blockers, and was inhibited by dipyridamole and stimulated by iloprost. Thus, in erythrocytes, two independent pathways mediate the release of ATP. We also show that glyburide is a strong inhibitor of Panx1 channels.

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DOI: 10.1016/j.febslet.2011.09.033

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