Noncovalent Labeling of Biomolecules with Red and Near- Infrared Dyes
Gabor PatonayDepartment of Chemistry, Georgia State University, University Plaza, Atlanta, Georgia 30303, USAJozef SaloňDepartment of Chemistry, Georgia State University, University Plaza, Atlanta, Georgia 30303, USAJohn SowellDepartment of Chemistry, Georgia State University, University Plaza, Atlanta, Georgia 30303, USALucjan StrękowskiDepartment of Chemistry, Georgia State University, University Plaza, Atlanta, Georgia 30303, USA
2004en
ABI
Annotatsiya
Biopolymers such as proteins and nucleic acids can be labeled with a fluorescent marker to allow for their detection. Covalent labeling is achieved by the reaction of an appropriately functionalized dye marker with a reactive group on a biomolecule. The recent trend, however, is the use of noncovalent labeling that results from strong hydrophobic and/or ionic interactions between the marker and biomolecule of interest. The main advantage of noncovalent labeling is that it affects the functional activity of the biomolecule to a lesser extent. The applications of luminescent cyanine and squarylium dyes are reviewed.
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