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Endophytic Actinobacteria Associated with<i> Dracaena cochinchinensis</i> Lour.: Isolation, Diversity, and Their Cytotoxic Activities

Nimaichand SalamState Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, ChinaThi-Nhan KhieuDepartment of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, VietnamMin‐Jiao LiuYunnan Institute of Microbiology, Yunnan University, Kunming 650091, ChinaThu-Trang VuDepartment of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, VietnamSon Chu‐KyDepartment of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, VietnamNgoc-Tung QuachLaboratory of Fermentation Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamQuyet‐Tien PhiLaboratory of Fermentation Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamManik Prabhu Narsing RaoState Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, ChinaAngélique FontanaCIRAD, UMR QUALISUD, 34398 Montpellier, FranceSamira SarterCIRAD, UMR QUALISUD, 34398 Montpellier, FranceWen‐Jun LiState Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China

2017en

ABI

Annotatsiya

Dracaena cochinchinensis Lour. is an ethnomedicinally important plant used in traditional Chinese medicine known as dragon’s blood. Excessive utilization of the plant for extraction of dragon’s blood had resulted in the destruction of the important niche. During a study to provide a sustainable way of utilizing the resources, the endophytic Actinobacteria associated with the plant were explored for potential utilization of their medicinal properties. Three hundred and four endophytic Actinobacteria belonging to the genera Streptomyces , Nocardiopsis , Brevibacterium , Microbacterium , Tsukamurella , Arthrobacter , Brachybacterium , Nocardia , Rhodococcus , Kocuria , Nocardioides , and Pseudonocardia were isolated from different tissues of D. cochinchinensis Lour. Of these, 17 strains having antimicrobial and anthracyclines-producing activities were further selected for screening of antifungal and cytotoxic activities against two human cancer cell lines, MCF-7 and Hep G2. Ten of these selected endophytic Actinobacteria showed antifungal activities against at least one of the fungal pathogens, of which three strains exhibited cytotoxic activities with IC 50 -values ranging between 3 and 33 μ g·mL −1 . Frequencies for the presence of biosynthetic genes, polyketide synthase- (PKS-) I, PKS-II, and nonribosomal peptide synthetase (NRPS) among these 17 selected bioactive Actinobacteria were 29.4%, 70.6%, and 23.5%, respectively. The results indicated that the medicinal plant D. cochinchinensis Lour. is a good niche of biologically important metabolites-producing Actinobacteria.

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Identifikatorlar

DOI: 10.1155/2017/1308563

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