Electrospray from Nanostructured Tungsten Oxide Surfaces with Ultralow Sample Volume
Jingyueh JengDepartment of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan, and Department of Mechanical and Electro-Mechanical Engineering, and Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, TaiwanChe‐Hsin LinDepartment of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan, and Department of Mechanical and Electro-Mechanical Engineering, and Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, TaiwanJentaie ShieaDepartment of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan, and Department of Mechanical and Electro-Mechanical Engineering, and Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan
2005en
ABI
Annotatsiya
This study demonstrated the feasibility of performing protein analysis with ultralow sample volume by combining a tungsten oxide nanowire (TON) fiber with a miniaturized electrospray ionization interface. An increase in wettability of the tugsten surface after growing randomly oriented TON on its surface allows strong adhesion of approximately 50 nL of the methanol solution at its tip. Under the influence of a high electric field, electrospray from a Taylor cone on the adhered methanol solution was observed and the multiply charged ions of protein molecules predissolved in the solution were detected.
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