Theoretical and experimental demonstration of resolution beyond the Rayleigh limit by FINCH fluorescence microscopic imaging
Joseph RosenDepartment of Biomedical Engineering, Johns Hopkins University, Rockville, Maryland 20850, USA. [email protected]Nisan SiegelDepartment of Biomedical Engineering, Johns Hopkins University, 9605 Medical Center Drive, Rockville, Maryland 20850, USAGary BrookerDepartment of Biomedical Engineering, Johns Hopkins University, 9605 Medical Center Drive, Rockville, Maryland 20850, USA
2011en
ABI
Annotatsiya
Fresnel Incoherent Correlation Holography (FINCH) enables holograms to be recorded from incoherent light with just a digital camera and spatial light modulator. We previously described its application to general three dimensional incoherent imaging and specifically to fluorescence microscopy, wherein one complex hologram contains the three dimensional information in the field of view, obviating the need for scanning or serial sectioning. We have now further analyzed FINCH in view of linear system theory and in comparison to conventional coherent and incoherent two dimensional imaging systems. We demonstrate, theoretically and experimentally, improved resolution by FINCH, when compared to conventional imaging.
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