Maqola

Direct Transverse Relaxation Time Biosensing Strategy for Detecting Foodborne Pathogens through Enzyme-Mediated Sol–Gel Transition of Hydrogels

Luyu WeiCollege of Food Science and Technology, Huazhong Agricultural University, Shizishan Street, Hongshan District, Wuhan 430070, Hubei, ChinaZhilong WangCollege of Food Science and Technology, Huazhong Agricultural University, Shizishan Street, Hongshan District, Wuhan 430070, Hubei, ChinaCaiwei FengYunlei XianyuCollege of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou 310058, Zhejiang, ChinaYiping ChenCollege of Food Science and Technology, Huazhong Agricultural University, Shizishan Street, Hongshan District, Wuhan 430070, Hubei, China

2021en

ABI

Annotatsiya

In this work, we develop a direct transverse relaxation time (T2) biosensing strategy and employ it for assaying foodborne pathogens relying on the alkaline phosphatase (ALP)-mediated sol–gel transition of hydrogels. ALP can catalyze the reaction to generate an acidic environment to transform the sol-state alginate solution to hydrogel, and this hydrogelation process can directly regulate the diffusion rate of water protons that results in a T2 change of water molecules. By means of enzyme-modulated sol–gel transition and antigen–antibody interactions, this T2 biosensor displays high sensitivity for detecting 50 CFU/mL S. enteritidis within 2 h. This biosensing strategy directly modulates the water molecules rather than magnetic probes in traditional methods, offering a straightforward, novel, and sensitive platform for pathogen detection.

Hali tarjima qilinmagan

Identifikatorlar

DOI: 10.1021/acs.analchem.0c03968

Iqtiboslar va manbalar

2 ta iqtibos0 ta foydalanilgan manba

Koʻrsatkichlar — AkademScholar