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Anti-Tumor Effect of Steamed Codonopsis lanceolata in H22 Tumor-Bearing Mice and Its Possible Mechanism

Wei LiCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaQi XuCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaYufang HeJilin Academy of Chinese Medicine Sciences, Changchun 130012, ChinaYing LiuCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaShubao YangCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, ChinaZi WangCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaJing ZhangCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, ChinaLichun ZhaoCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530011, China

2015en

ABI

Annotatsiya

Although previous studies confirmed that steaming and the fermentation process could significantly improve the cognitive-enhancement and neuroprotective effects of Codonopsis lanceolata, the anti-tumor efficacy of steamed C. lanceolata (SCL) and what mechanisms are involved remain largely unknown. The present study was designed to evaluate the anti-tumor effect in vivo of SCL in H22 tumor-bearing mice. The results clearly indicated that SCL could not only inhibit the tumor growth, but also prolong the survival time of H22 tumor-bearing mice. Besides, the serum levels of cytokines, such as interferon gamma (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-2 (IL-2), were enhanced by SCL administration. The observations of Hoechst 33258 staining demonstrated that SCL was able to induce tumor cell apoptosis. Finally, immunohistochemical analysis revealed that SCL treatment significantly increased Bax expression and decreased Bcl-2 and vascular endothelial growth factor (VEGF) expression of H22 tumor tissues in a dose-dependent manner. Moreover, LC/MS analysis of SCL indicated that it mainly contained lobetyolin and six saponins. Taken all together, the findings in the present study clearly demonstrated that SCL inhibited the H22 tumor growth in vivo at least partly via improving the immune functions, inducing apoptosis and inhibiting angiogenesis.

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Identifikatorlar

DOI: 10.3390/nu7105395

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