A Modified CTAB Protocol for Plant DNA Extraction
Jinlu Li1College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China;
2State Key Laboratory of Systematic & Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaShuo Wang1College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China;
2State Key Laboratory of Systematic & Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaJing Yu1College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China;
2State Key Laboratory of Systematic & Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaLing Wang1College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China;
2State Key Laboratory of Systematic & Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaShiliang Zhou1College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China;
2State Key Laboratory of Systematic & Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
2013en
ABI
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摘要： 植物组织中含有大量多糖、多酚、酯类等次生代谢产物, 要从中提取高质量的DNA比较困难。针对这一情况, 该文提出一种改良CTAB植物DNA提取方法(mCTAB), 并以10种常见植物为实验材料, 与4种常用的植物DNA提取试剂盒作对比。结果表明, mCTAB法提取的DNA产率高且质量好, PCR扩增成功率也较高, 而提取成本显著低于DNA提取试剂盒, 可有效用于植物DNA条形码等研究的植物DNA提取。
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