Alantolactone induces apoptosis and suppresses migration in MCF‑7 human breast cancer cells via the p38�MAPK, NF‑κB and Nrf2 signaling pathways

Human breast cancer is a malignant type of cancer with high prevalence. In the present study, the anticancer effects of alantolactone, a sesquiterpene lactone, on the human breast cancer cell line MCF‑7 were investigated in vitro. The MCF‑7 cell morphology changed from diamond to round subsequent to treatment with alantolactone, and the cell viability reduced significantly compared with that of the control cells. Alantolactone induced apoptosis of MCF‑7 cells by regulating the protein expression levels of B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein, p53, caspase‑3 and caspase‑12, which are associated with the apoptotic pathway, and suppressed colony formation and migration by regulating the protein expression of matrix metalloproteinase (MMP)‑2, MMP‑7 and MMP‑9. Cell signaling pathway analysis confirmed that alantolactone increased the phosphorylation of p38, and decreased the nuclear expression levels of p65 and nuclear factor erythroid 2‑related factor 2 (Nrf2), suggesting that the apoptosis‑promoting and migration‑suppressing effect of alantolactone may partially depend on regulating the p38 MAPK, NF‑κB and Nrf2 pathways. These results also suggested that alantolactone may become a potential therapeutic strategy for treating breast cancer.

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