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Tissue Engineered Bio‐Blood‐Vessels Constructed Using a Tissue‐Specific Bioink and 3D Coaxial Cell Printing Technique: A Novel Therapy for Ischemic Disease

Ge GaoDepartment of Mechanical Engineering Pohang University of Science and Technology Pohang 37673 Republic of KoreaJun Hee LeeDepartment of Pharmacology and Toxicology University of Alabama at Birmingham School of Medicine Birmingham AL 35294 USAJinah JangDepartment of Creative IT Engineering Pohang University of Science and Technology Pohang 37673 Republic of KoreaDong‐Han LeeLaboratory for Vascular Medicine and Stem Cell Biology Medical Research Institute Department of Physiology School of Medicine Pusan National University Yangsan 626‐870 Republic of KoreaJeong‐Sik KongSchool of Interdisciplinary Bioscience and Bioengineering Pohang University of Science and Technology Pohang 37673 Republic of KoreaByoung Soo KimDepartment of Mechanical Engineering Pohang University of Science and Technology Pohang 37673 Republic of KoreaYeong‐Jin ChoiDivision of Integrative Biosciences and Biotechnology Pohang University of Science and Technology Pohang 37673 Republic of KoreaWoong Bi JangLaboratory for Vascular Medicine and Stem Cell Biology Medical Research Institute Department of Physiology School of Medicine Pusan National University Yangsan 626‐870 Republic of KoreaYoung Joon HongHeart Center of Chonnam National University Hospital Gwangju 501‐757 Republic of KoreaSang‐Mo KwonLaboratory for Vascular Medicine and Stem Cell Biology Medical Research Institute Department of Physiology School of Medicine Immunoregulatory Therapeutics Group in Brain Busan 21 Project Pusan National University Yangsan 626‐870 Republic of KoreaDong‐Woo ChoDepartment of Mechanical Engineering Pohang University of Science and Technology Pohang 37673 Republic of Korea

2017en

ABI

Annotatsiya

Endothelial progenitor cells (EPCs) are a promising cell source for the treatment of several ischemic diseases for their potentials in neovascularization. However, the application of EPCs in cell‐based therapy has shown low therapeutic efficacy due to hostile tissue conditions after ischemia. In this study, a bio‐blood‐vessel (BBV) is developed, which is produced using a novel hybrid bioink (a mixture of vascular‐tissue‐derived decellularized extracellular matrix (VdECM) and alginate) and a versatile 3D coaxial cell printing method for delivering EPC and proangiogenic drugs (atorvastatin) to the ischemic injury sites. The hybrid bioink not only provides a favorable environment to promote the proliferation, differentiation, and neovascularization of EPCs but also enables a direct fabrication of tubular BBV. By controlling the printing parameters, the printing method allows to construct BBVs in desired dimensions, carrying both EPCs and atorvastatin‐loaded poly(lactic‐ co ‐glycolic) acid microspheres. The therapeutic efficacy of cell/drug‐laden BBVs is evaluated in an ischemia model at nude mouse hind limb, which exhibits enhanced survival and differentiation of EPCs, increased rate of neovascularization, and remarkable salvage of ischemic limbs. These outcomes suggest that the 3D‐printed ECM‐mediated cell/drug implantation can be a new therapeutic approach for the treatment of various ischemic diseases.

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Identifikatorlar

DOI: 10.1002/adfm.201700798

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