Development of an ancestral DC and TLR4-inducing multi-epitope peptide vaccine against the spike protein of SARS-CoV and SARS-CoV-2 using the advanced immunoinformatics approaches
Cena AramDepartment of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranParsa AlijanizadehStudent Research Committee, Babol University of Medical Sciences, Babol, IranKiarash SalekiStudent Research Committee, Babol University of Medical Sciences, Babol, IranLeila KaramiDepartment of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
2024en
ABI
Annotatsiya
I position of the pET-28a (+) vector, and simulating the agarose gel revealed the existence of the inserted gene in the cloned plasmid with SARS vaccine (SARSV) construct, which has a 6565 bp in length overall. In terms of cytokines/IgG response, immunological simulation revealed a strong immune response. The stabilized vaccine showed strong interactions with TLR3/4, according to Molecular Dynamics Simulation (MDS) analysis. The present ancestral vaccine targets common sequences which seem to be valuable targets even for the new variant SARS-CoV-2.
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