Generation of multiply charged peptides and proteins by radio frequency acoustic desorption and ionization for mass spectrometric detection
R. Brent DixonW. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, 27695, Raleigh, NC, USAJason S. SampsonW. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, 27695, Raleigh, NC, USADavid C. MuddimanW. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, 27695, Raleigh, NC, USA
2008en
ABI
Annotatsiya
The design and implementation of a radio frequency acoustic desorption ionization (RADIO) source has been demonstrated for the analysis of multiply charged peptides and proteins. One muL aliquots of melittin, BNP-32, and ubiquitin ( approximately 1 mug of analyte) were deposited onto a quartz crystal microbalance (QCM) electrode before radio frequency actuation for desorption. Continuous electrospray parallel to/above the sampling surface enabled the ionization of desorbed species. Detection by a hybrid linear ion trap Fourier transform ion cyclotron resonance mass spectrometer confirmed the intact and dissociated species observed during MS and MS/MS experiments, respectively.
Hali tarjima qilinmagan
Identifikatorlar
Iqtiboslar va manbalar
3 ta iqtibos0 ta foydalanilgan manba