Massive cluster impact mass spectrometry: A new desorption method for the analysis of large biomolecules
John F. MahoneyPhrasor Scientific Inc., Duarte, CA 91010Julius PerelPhrasor Scientific Inc., 1536 Highland Avenue, Duarte, CA 91010, USAStephen A. RuattaPhrasor Scientific Inc., 1536 Highland Avenue, Duarte, CA 91010, USAP. A. MartinoDivision of immunology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USAS. Waqif HusainDivision of immunology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USAKelsey D. CookDepartment of Chemistry, University of Tennessee, TN, USAT D LeeDivision of immunology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
1991en
ABI
Annotatsiya
A new ion desorption method is described that utilizes a primary beam of massive, multiply charged cluster ions to generate secondary ions of peptides in a glycerol matrix. The massive cluster ion beam is generated via electrohydrodynamic emission using a 1.5 M solution of ammonium acetate in 30% aqueous glycerol. Negative ion spectra of peptides obtained using this technique show greatly decreased relative intensities for fragment ions and 'chemical noise' background when compared to spectra obtained using a xenon atom primary beam. The near absence of fragments derived from radiation damage to the sample solution is attributed to the impact of primary particles with energies less than 1 eV/nucleon.
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