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Hybrid <i>de novo</i> genome assembly of Chinese chestnut (<i>Castanea mollissima</i>)

Yu XingBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,Yang LiuCollege of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,Qing ZhangCollege of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,Xinghua NieCollege of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,Yamin SunResearch Center for Functional Genomics and Biochip , 23 Hongda St., Tianjin 300457,Zhiyong ZhangBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,Huchen LiBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,Kefeng FangCollege of Landscape Architecture, Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,Guangpeng WangChangli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences , 39 E Jieyangdajie, Changli 066600,Hongwen HuangSouth China Botanical Garden, Chinese Academy of Sciences , 723 Xingke Rd., Guangzhou 510650,Ton BisselingBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,Qingqin CaoBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,Ling QinBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture , 7 Beinong Rd., Beijing 102206,
2019en
ABI

Annotatsiya

BACKGROUND: The Chinese chestnut (Castanea mollissima) is widely cultivated in China for nut production. This plant also plays an important ecological role in afforestation and ecosystem services. To facilitate and expand the use of C. mollissima for breeding and its genetic improvement, we report here the whole-genome sequence of C. mollissima. FINDINGS: We produced a high-quality assembly of the C. mollissima genome using Pacific Biosciences single-molecule sequencing. The final draft genome is ∼785.53 Mb long, with a contig N50 size of 944 kb, and we further annotated 36,479 protein-coding genes in the genome. Phylogenetic analysis showed that C. mollissima diverged from Quercus robur, a member of the Fagaceae family, ∼13.62 million years ago. CONCLUSIONS: The high-quality whole-genome assembly of C. mollissima will be a valuable resource for further genetic improvement and breeding for disease resistance and nut quality.

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