Virion RNA of Beet Yellows Closterovirus: Cell-free Translation and Some Properties
Alexander V. KarasevInstitute of Microbiology, 117811 MoscowA. A. AgranovskyDepartment of Virology and A. N. Belozersky Laboratory at Moscow State University, 119899 Moscow, U.S.S.RВ. В. РоговDepartment of Virology and A. N. Belozersky Laboratory at Moscow State University, 119899 Moscow, U.S.S.RN.A. MiroshnichenkoInstitute of Microbiology, 117811 MoscowV. V. DoljaDepartment of Virology and A. N. Belozersky Laboratory at Moscow State University, 119899 Moscow, U.S.S.RJ.G. AtabekovDepartment of Virology and A. N. Belozersky Laboratory at Moscow State University, 119899 Moscow, U.S.S.R
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SUMMARY Virion RNA of beet yellows virus (BYV) is a messenger-sense RNA of about 14500 nucleotides containing no poly(A) or covalently linked protein. In υitro translation of BYV RNA yielded a prominent polypeptide of M r 250000, and some lighter products. None of the translation products was found to be immunoprecipitable with anti-BYV γ-globulins. The synthesis of all the BYV RNA-directed polypeptides was blocked by the cap analogue m7Gpp, thus suggesting the presence of a cap structure at the 5′ end of virion RNA.
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