Aflatoxin-free transgenic maize using host-induced gene silencing
Dhiraj ThakareBIO5 Institute, School of Plant Sciences, University of Arizona, 1657 E. Helen Street, Tucson, AZ 85721, USAJianwei ZhangArizona Genomics Institute, 1657 E. Helen Street, Tucson, AZ 85721, USARod A. WingArizona Genomics Institute, 1657 E. Helen Street, Tucson, AZ 85721, USAPeter J. CottyAgricultural Research Service, U.S. Department of Agriculture, and School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USAMonica A. SchmidtBIO5 Institute, School of Plant Sciences, University of Arizona, 1657 E. Helen Street, Tucson, AZ 85721, USA
2017en
ABI
Annotatsiya
aflatoxin biosynthetic pathway. After pathogen infection, aflatoxin could not be detected in kernels from these RNAi transgenic maize plants, while toxin loads reached thousands of parts per billion in nontransgenic control kernels. A comparison of transcripts in developing aflatoxin-free transgenic kernels with those from nontransgenic kernels showed no significant differences between these two groups. These results demonstrate that small interfering RNA molecules can be used to silence aflatoxin biosynthesis in maize, providing an attractive and precise engineering strategy that could also be extended to other crops to improve food security.
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