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Desorption of cellulases from cotton powder

Helena S. AzevedoDepartment of Textile Engineering, Minho University, 4800-058, Guimarães, PortugalLuiz Pereira RamosResearch Center in Applied Chemistry, Department of Chemistry, Federal University of Paraná, P.O. Box, 19081, Curitiba, 81531-990, BrazilArtur Cavaco‐PauloDepartment of Textile Engineering, Minho University, 4800-058, Guimarães, Portugal

2001en

ABI

Annotatsiya

Cotton fabrics were treated with three different Trichoderma reesei cellulase preparations (total crude – TC, endoglucanase enriched – EG-rich, cellobiohydrolase enriched – CBH-rich) using mechanical agitation to produce cotton powder. Desorption of cellulase enzymes from the cotton powder was then performed by washing with buffer. After 3 washings most of the protein was desorbed from the cotton powder and the amount of sugars released in the latter washings was negligible. TC and CBH-rich preparations produced a finer cellulose powder than EGs. The desorption process caused a decrease in degree of polymerisation (DP) specially for the cotton treated with EGs and a marked increase in polydispersity (P d) for all preparations.

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Identifikatorlar

DOI: 10.1023/a:1011619425613

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