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Direct Gene Transfer into Mouse Muscle in Vivo

Jon A. WolffDepartments of Pediatrics and Genetics, Waisman Center, University of Wisconsin, Madison, WI 53706Robert W. MaloneVical Inc., San Diego, CA 92121Phillip WilliamsDepartments of Pediatrics and Genetics, Waisman Center, University of Wisconsin, Madison, WI 53706Chong WangDepartments of Pediatrics and Genetics, Waisman Center, University of Wisconsin, Madison, WI 53706Gyula AcsádiDepartments of Pediatrics and Genetics, Waisman Center, University of Wisconsin, Madison, WI 53706Ágnes JániDepartments of Pediatrics and Genetics, Waisman Center, University of Wisconsin, Madison, WI 53706Philip L. FelgnerVical Inc., San Diego, CA 92121

1990en

ABI

Annotatsiya

RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for beta-galactosidase activity was localized to muscle cells following injection of the beta-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.

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DOI: 10.1126/science.1690918

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