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Snake Venomic of <i>Crotalus durissus terrificus</i>—Correlation with Pharmacological Activities

Dessislava GeorgievaInstitute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestrasse 85, Building 22a, 22603 Hamburg, Germany, Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permose Strasse 15, 04318 Leipzig, Germany, Department of Physics, IBILCE/UNESP, Cristóvão Colombo 2265, CEP 15054-000, São José do Rio Preto, SP Brazil, and Institute of Organic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia,Michaela ÖhlerInstitute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestrasse 85, Building 22a, 22603 Hamburg, Germany, Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permose Strasse 15, 04318 Leipzig, Germany, Department of Physics, IBILCE/UNESP, Cristóvão Colombo 2265, CEP 15054-000, São José do Rio Preto, SP Brazil, and Institute of Organic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia,Jana SeifertInstitute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestrasse 85, Building 22a, 22603 Hamburg, Germany, Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permose Strasse 15, 04318 Leipzig, Germany, Department of Physics, IBILCE/UNESP, Cristóvão Colombo 2265, CEP 15054-000, São José do Rio Preto, SP Brazil, and Institute of Organic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia,Martin von Bergen�Institute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestrasse 85, Building 22a, 22603 Hamburg, Germany, Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permose Strasse 15, 04318 Leipzig, Germany, Department of Physics, IBILCE/UNESP, Cristóvão Colombo 2265, CEP 15054-000, São José do Rio Preto, SP Brazil, and Institute of Organic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia,Raghuvir K. ArniInstitute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestrasse 85, Building 22a, 22603 Hamburg, Germany, Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permose Strasse 15, 04318 Leipzig, Germany, Department of Physics, IBILCE/UNESP, Cristóvão Colombo 2265, CEP 15054-000, São José do Rio Preto, SP Brazil, and Institute of Organic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia,Nicolay GenovInstitute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestrasse 85, Building 22a, 22603 Hamburg, Germany, Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permose Strasse 15, 04318 Leipzig, Germany, Department of Physics, IBILCE/UNESP, Cristóvão Colombo 2265, CEP 15054-000, São José do Rio Preto, SP Brazil, and Institute of Organic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia,Christian BetzelInstitute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestrasse 85, Building 22a, 22603 Hamburg, Germany, Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permose Strasse 15, 04318 Leipzig, Germany, Department of Physics, IBILCE/UNESP, Cristóvão Colombo 2265, CEP 15054-000, São José do Rio Preto, SP Brazil, and Institute of Organic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia,

2010en

ABI

Annotatsiya

The snake venomic of Crotalus durissus terrificus was analyzed by 2-D and 1-D electrophoresis and subsequent MS/MS and enzymatic assays. The venomic of the South American rattlesnake comprises toxins from seven protein families: phospholipases A(2), serine proteinases, ecto-5'-nucleotidases, metalloproteinases, nerve growth factors, phosphodiesterases, and glutaminyl cyclase. The venom toxin composition correlates with the clinical manifestation of the crotalinae snake bites and explains pathological effects of the venom such as neurotoxicity, systemic myonecrosis, hemostatic disorders, myoglobinuria, and acute renal failure. The vast majority of toxins are potentially involved in neurotoxicity, myotoxicity, and coagulopathy. The predominant venom components are neurotoxic phospholipases A(2) and serine proteinases. The venom is a rich source of 5'-nucleotidases (7.8% of the identified toxins) inducing hemostatic disorders. Analysis of the venom protein composition provided a catalogue for secreted toxins. The venomic composition of Crotalus d. terrificus and venom gland transcriptome of the synonymous subspecies Crotalus d. collilineatus show differences in the occurrence of protein families and in the abundance of toxins. Some of the venom components identified by the proteomic analysis were not reported in the transcriptome of the Crotalus d. collilineatus venom gland. Enzymatic activities of the Crotalus d. terrificus venom were determined and correlated with the proteomic composition.

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DOI: 10.1021/pr901042p

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