Real time quantitative PCR.
Chris HeidBioAnalytical Technology Department, Genentech, Inc., South San Francisco, California 94080, USAJunko StevensBioAnalytical Technology Department, Genentech, Inc., South San Francisco, California 94080, USAKenneth J. LivakBioAnalytical Technology Department, Genentech, Inc., South San Francisco, California 94080, USAP. WilliamsBioAnalytical Technology Department, Genentech, Inc., South San Francisco, California 94080, USA
1996en
ABI
Annotatsiya
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
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