Study on Industrialization Tissue Culture Seedlings Technology of Cassava

Abstract

Taking 'NE199' and 'the Qinzhou local' cassava as experiment materials to study industrialization tissue culture seedlings technology.The results showed that:The micro-cuttage medium using MS+BA 0.06~0.08 mg·L-1+NAA 0.02 mg·L-1+GA3 0.02 mg·L-1,light intensity of 1 500~3 000 1x and incubation temperature of 25~30℃,the propagation coefficient could up to 4.0~4.2,axillary bud sprouting rate could reach above 90%,the vitrification rate and leaf yellowing rate could be controlled within 2% and 6%.With the cultivation loess∶sand=8∶1,the transplanting survival rate could reach more than 90%,which could achieve the industrialization of cassava plant tissue culture.

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