Article

Convalescent serum-derived exosomes: Attractive niche as COVID-19 diagnostic tool and vehicle for mRNA delivery

Anand KrishnanDepartment of Chemical Pathology, School of Pathology, Faculty of Health Sciences, University of the Free State, Bloemfontein 9300, South AfricaPrakash GangadaranBK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu 41944, Republic of KoreaVivek P. ChavdaDepartment of Pharmaceutics and Pharmaceutical Technology, L. M. College of Pharmacy, Ahmedabad 380009, IndiaManasi P. JogalekarHelen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, CA 94158, USARamesh MuthusamyDisha ValuResearch and Development, Intas Pharmaceuticals Ltd. (Biopharma Division), Ahmedabad 382213, IndiaChithravel VadivalaganMolecular Cell Physiology Laboratory, Department of Biochemistry, School of Medicine, AKFA University, Tashkent 100042, UzbekistanPrasanna RamaniCenter of Excellence in Advanced Materials & Green Technologies (CoE–AMGT), Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore 641112, IndiaA.I. LaishevtcevFederal Research Center—All-Russian Scientific Research Institute of Experimental Veterinary Medicine named after K.I. Skryabin and Y.R. Kovalenko of the Russian Academy of Sciences, Moscow 117218, RussiaNaresh Kumar KatariDepartment of Chemistry, GITAM (Deemed to be University), Hyderabad 502329, IndiaByeong‐Cheol AhnBK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea

Experimental Biology and Medicinejournal2022en

ABI

Abstract

The spread of SARS-CoV-2 over the entire world is more commonly known as COVID-19. COVID-19 has impacted society in every aspect of routine life. SARS-CoV-2 infection is often misdiagnosed as influenza or seasonal upper respiratory tract viral infections. General diagnostic tools can detect the viral antigen or isotypes of antibodies. However, inter- and intraindividual variations in antibody levels can cause false negatives in antibody immunoassays. On the contrary, the false-positive test results can also occur due to either cross-reactivity of the viral antigens or some other patient-related autoimmune factors. There is need for a cogent diagnostic tool with more specificity, selectivity, and reliability. Here, we have described the potential of convalescent serum-derived exosome as a diagnostic tool for the detection of SARS-CoV-2, even in asymptomatic patients, which is a limitation for currently practiced diagnostic tests throughout the globe. In addition, its potential as a vehicle for messenger RNA (mRNA) delivery is also emphasized.

Topics

Extracellular vesicles in disease SARS-CoV-2 and COVID-19 Research SARS-CoV-2 detection and testing

Identifiers

DOI: 10.1177/15353702221092984

Citations and references

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