The diameter of water pores formed by colicin Ia in planar lipid bilayers.
Krasilnikov OvLaboratory of Molecular Physiology, Institute of Physiology and Biophysics, Tashkent, UzbekistanYuldasheva LnTashkent Pediatric Medical Institute, Department of Biochemistry, Tashkent, UzbekistánNogueira RaUniversidade Federal de Pernambuco, Departamento de Biofisica e Radiobiologia, Recife, Pernambuco, BrasilRodrigues CgUniversidade Federal de Pernambuco, Departamento de Biofisica e Radiobiologia, Recife, Pernambuco, Brasil
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Abstract
The effective size of colicin Ia channel was tested by a recently described method (FEMS, Microbiology and Immunology (1992). 105: 93-100) in which the nonelectrolyte molecules with different hydrodynamic diameters (0.52 to 5.0 nm) were used as molecular tools. We have shown that despite low conductance (55-105 pS at 1.5 M KCl, pH 7.0) the ion channels formed by colicin Ia have a fairly large water pore diameter equal to 1.66-1.88 nm. The results are discussed in terms of an energetic barrier for ions passing into the channel lumen.
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